Published by the American Society for Testing and Materials (ASTM), astm e2149 describes antibacterial activity properties under dynamic contact conditions and how antibacterial agents are tested. The official names of this standard are astm e2149. Standard test method for measuring the antibacterial activity of antibacterial agents under dynamic contact conditions.
Immobilized antibacterial agents are chemically bound and cannot freely diffuse into the environment under normal use conditions. Therefore, fiber test methods such as AATCC147-2004, which directly rely on the easy exudation of antimicrobial agents from treated fabrics, are unsuitable for assessing immobilized antimicrobial agents. This test method ensures good contact between the bacteria and the treated fiber, cloth, or another substrate by constantly stirring the test sample in the challenge suspension during the test period. The metabolic status of the challenge species can directly affect the measurement of the effectiveness of a particular antibiotic or drug concentration. In addition, the susceptibility of a species to a particular biocide can vary depending on its life stage (cycle). One hour of contact time in buffer allows for metabolic stagnation in the population. This test method standardizes both the growth conditions of the challenge species and the contact time with the substrate to reduce variability associated with the growth phase of the microorganism. Liquid analysis of the antibacterial activity of non-leaching agents provides the ability to wet the test substrate thoroughly. Wet surfactants can reduce the false negatives observed when comparing hydrophobic and hydrophilic substrates. ASTM E2149 test method This test is suitable for evaluating stressed or modified samples with proper control. This astm e2149 test method is designed to assess the resistance of antibacterial treated samples without leaching into microbial growth under dynamic contact conditions. This dynamic shaking flask test was developed for routine quality control and screening tests to overcome the difficulty of using classic antibacterial test methods to evaluate substrate-bound antibacterial agents. It was done. These difficulties include ensuring contact of the inoculum with the treated surface (for AATCC 100), the flexibility of recovery at various contact times, and improperly applied static conditions (AATCC 147. • The test also allows for various tests for hard water, proteins, blood, serum, multiple chemicals, other contaminants, or contamination due to physical/chemical stress or manipulation of the target sample. • Surface antibacterial activity is determined by comparing the results of test samples with controls performed concurrently. • The presence of exudative antibiotics is pre-determined and then determined by the presence of suppression zones. • This test method should only be performed by those who have been trained in microbiological techniques. • This standard may include dangerous goods, operations, and equipment. This standard is not intended to address all safety concerns associated with its use. Users of this standard are responsible for establishing good health and safety practices and determining the applicability of regulatory restrictions before use.
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